Analysis of amylose content in sweet corn starch (Zea Mays L. Var. Saccharata) by UV-Vis spectrophotometry / Analisis kadar amilosa pada pati jagung manis (Zea Mays L. Var. Saccharata) secara spektrofotometri UV-Vis

Sweet Corn Starch (Zea mays L. var. saccharata) is composed of two fractions: amylose and amylopectin. The determination of amylose content in Sweet Corn Starch is carried out using the spectrophotometric method. This method employs a 2% iodine solution as a reagent, which reacts with starch to generate a coloured solution that can be measured using a spectrophotometer. The amylose content is quantified through visible spectrophotometry, resulting in a regression equation of y ̂ = 0.006 + 25.2x, with a coefficient of determination (R2) = 0.998 and a correlation coefficient (r) = 0.9989. The amylose content in Sweet Corn Starch is determined to be 28.65%, whereas the amylopectin content is found to be 71.35%. Furthermore, the analysis provides additional parameters: Standard Deviation (SD) = ± 0.0044, Detection Limit (BD) = 0.0005 mg/ml, and Quantity Limit (BK) = 0.0018 mg/ml. These parameters offer valuable insights into the quality and properties of the sweet corn starch sample.

Pati Jagung Manis (Zea mays L. var. saccharata) terdiri dari dua fraksi yaitu amilosa dan amylopectin. Penentuan kadar amilosa pada Pati Jagung Manis (Zea mays L. var. saccharata)  dapat ditentukan menggunakan metode spektrofotometri. Kadar amilosa ditetapka dengan metode spektrofotometri visible dengan pereaksi larutan iod 2% yang dapat bereaksi dengan amilum membentuk larutan bewarna yang dapat diukur dengan alat spetrofotometer. Dari pengukuran absorban diperoleh Persamaan regresi  = 0,006+ 25,2x, dengan nilai koefisien determinasi (R2) =  dan nilai koefisien kolerasi (r) = . Kadar amilosa yang diperoleh dari pati Jagung Manis (Zea mays L. var. saccharata) yaitu 28,65%  dan untuk kadar amilopektin dari pati jagung manis sebesar 71,35% dengan Standar Deviasi (SD) = ± 0,0044, Batas Deteksi (BD) = 0,0005 mg/ml dan Batas Kuntitas (BK) = 0,0018 mg/ml.