Activity Test of Ethanol Extract from Chayote Leaves (Sechium edule Jacq. Swartz) as an Antibacterial against Mycobacterium smegmatis / Uji Aktivitas Ekstrak Etanol Daun Labu Siam (Sechium edule Jacq. Swartz) sebagai Antibakteri Mycobakterium smegmatis

The membrane structure of Mycobacterium smegmatis is similar to that of Mycobacterium tuberculosis, allowing TB testing to be performed on Mycobacterium smegmatis without directly testing on Mycobacterium tuberculosis. Antibacterial testing uses the paper disc method with several treatments, namely extract concentrations of 1 µg/disc, 2 µg/disc, and 4 µg/disc, as well as positive control streptomycin 2 µg/disc and negative control 70% ethanol. The research results obtained at Extract Concentrations of 1, 2, and 4 µg/disc have moderate antibacterial activity seen from the inhibitory zone diameter, namely 8.73 mm, 9.17 mm, and 9.73 mm, and the positive control is strong because it has a zone diameter of 13.27 and the negative control does not give a zone. The conclusion is that the extract of siam pumpkin leaves has moderate antibacterial activity.

Struktur membran dari Mycobacterium smegmatis mirip dengan spesies Mycobacterium tuberculosis, sehingga pengujian TB bisa diuji pada Mycobacterium smegmatis tanpa harus diuji langsung pada Mycobacterium tuberculosis. Pengujian antibakteri menggunakan metode kertas cakram dengan beberapa perlakuan yaitu konsentrasi ekstrak 1 µg/disc, 2 µg/disc dan 4 µg/disc serta pembanding control positif streptomycin 2 µg/disc dan kontrol negatif etanol 70%. Hasil penelitian yang diperoleh  pada Konsentrasi Ekstrak 1,2 dan 4 µg/disc memiliki aktivitas antibakteri kategori sedang dilihat dari diameter zona hambat yaitu 8.73 mm, 9.17 mm dan 9,73 mm dan kontrol positif kategori kuat karena memiliki diameter zona 13,27 dan kontrol negatif tidak memberikan zona. Kesimpulan bahwa Ekstrak daun labu siam memberikan aktivitas antibakteri pada kategori sedang.