Determination of total flavonoid ethanol extract of jasmine leaf (Jasminum sambac (L.) Sol. ex Aiton) using spectrophotometric Uv-Vis method / Penetapan kadar flavonoid total ekstrak etanol daun bunga melati (Jasminum sambac (L.) Sol. ex Aiton) dengan spektrofotometri Uv-Vis

Traditional medicine uses plants with a natural ingredient content as its raw material. The bioactive compounds found in plants are secondary metabolites such as alkaloids, flavonoids, tannins, glycosides, steroids, and saponins. One of the plants containing secondary metabolite compounds is jasmine. This study aims to determine the chemical compounds contained in the ethanol extract and the total flavonoid content of the ethanol extract of jasmine leaves using the UV-Vis spectrophotometric method. The stages of this research included processing plant simplicia, making ethanol extract, examining characteristics, screening phytochemicals, and determining the total flavonoid content of the ethanol extract of jasmine leaves using the UV-Vis spectrophotometric method. Extract jasmine flower leaves by maceration method using 96% ethanol solvent; the extract obtained was concentrated using a rotary evaporator; then the total flavonoid content was determined using the UV-Vis spectrophotometry method. The research shows that the ethanol extract of jasmine flower leaves (Jasminum sambac (L.) Sol. ex Aiton) contains several secondary metabolite compounds, such as flavonoids, glycosides, alkaloids, saponins, steroids, and tannins. A number of steps were taken to find out how many flavonoids were in the ethanol extract as a whole. These included finding the maximum wavelength of quercetin, the operational time, making a quercetin calibration curve, and using UV-Vis spectrophotometry to determine how many flavonoids were in the extract. The analysis results reveal that the total flavonoid content in the ethanol extract of jasmine flower leaves is approximately 40.10911 ± 0.5878 mg QE/g.

Obat tradisional merupakan obat yang menggunakan tumbuhan dengan kandungan bahan alami sebagai bahan bakunya. Senyawa bioaktif yang terdapat pada tumbuhan berupa metabolit sekunder yaitu alkaloid, flavonoid, tanin, glikosida, steroid dan saponin. Tumbuhan yang mengandung senyawa metabolit sekunder salah satunya yaitu bunga melati. Tujuan dari penelitian yang dilakukan ini yaitu untuk mengetahui senyawa metabolit sekunder yang terkandung dan untuk mengetahui kadar flavonoid total dalam ekstrak etanol daun bunga melati dengan metode spektrofotometri UV-Vis. Tahapannya yaitu pengolahan simplisia, pembuatan ektrak etanol, pengujian karakteristik, pengujian skrining fitokimia serta penetapan kadar flavonoid total ekstrak etanol daun bunga melati metode spektofotometri UV-Vis. Ekstrak daun bunga melati menggunakan metode maserasi dengan pelarut etanol 96%, maserat dipekatkan menggunakan rotary evaporator, kemudian penetapan kadar flavonoid total menggunakan metode spektrofotometri UV-Vis. Hasil penelitian menunjukkan bahwa ekstrak etanol dari daun bunga melati (Jasminum sambac (L.) Sol. ex Aiton) mengandung beragam senyawa metabolit sekunder, termasuk flavonoid, glikosida, alkaloid, saponin, steroid dan tanin. Untuk menetapkan kadar flavonoid total dalam ekstrak etanol tersebut, dilakukan serangkaian tahapan, meliputi penentuan panjang gelombang maksimum kuersetin, waktu operasi, pembuatan kurva kalibrasi kuersetin, serta perhitungan kadar flavonoid total menggunakan metode spektrofotometri UV-Vis. Hasil analisis menunjukkan bahwa kadar flavonoid total dalam ekstrak etanol daun bunga melati adalah sekitar 40,10911 ± 0,5878 mg QE/g.