Antidiabetic test of combination of insulin leaf extracts (Tithonia diversifolia (Hems.) A.Gray) and purple sweet tattoo (Ipomoea batatas (L.) Lam) leaf in male rats due to streptozotocin induction / Uji antidiabetes kombinasi ekstrak daun insulin (Tithonia diversifolia (Hems.) A.Gray) dan daun ubi jalar (Ipomoea batatas (L.) Lam) ungu pada tikus jantan akibat induksi streptozotocin

Diabetes mellitus is a condition in which blood glucose levels are high enough in the body. Insulin leaves contain tannins, flavonoids and saponins which play a role in fighting free radicals. Purple sweet potato leaves have anthocyanin and phenolic flavonoid compounds that function as anti-diabetics. The combination of the two is expected to produce a synergistic effect to increase the effectiveness of antidiabetics. This research aims to find out the antidiabetic effect and the effective dose of a combination of insulin leaf extract and purple sweet potato leaves. This study used 25 rats as test animals which were divided into 5 groups. The blood glucose levels of the rats were measured using the GOD-PAP method. The results showed that the combination dose of insulin leaf ethanol extract 30 mg/kgBB and purple sweet potato leaves 120 mg/kgBB (combination group 2) had an anti-diabetic effect, an average of 97.49 and a positive control (glibenclamide) mean of 116.83 in reducing blood glucose levels in streptozotocin-induced mice. The research show that based on the One Way ANOVA analysis, the sig. ie 0.000 <0.05, then in conclusion the average of the five test groups has a significant difference.

Diabetes mellitus keadaan dimana kadar glukosa darah yang cukup tinggi didalam tubuh. Daun insulin memiliki kandungan tannin, flavonoid dan saponin yang berperan dalam melawan radikal bebas. Daun ubi jalar ungu miliki senyawa flavonoid antosianin, dan fenolik yang berfungsi sebagai antidiabetes. Gabungan kedua itu bisa menghasilkan efek sinergis guna tingkatkan efektifitas antidiabetes. Studi ini bertujuan guna mencari tahu adanya efek antidiabetes dan dosis efektif kombinasi ekstrak daun insulin dan daun ubi jalar ungu. Studi ini memakai hewan uji tikus ada 25 tikus yang terbagi jadi 5 kelompok, kadar glukosa darah tikus diukur memakai metode GOD-PAP. Hasil menunjukkan bahwa dosis kombinasi ekstrak etanol dau insulin 30 mg/kgBB dan daun ubi jalar ungu 120 mg/kgBB (kelompok kombinasi 2) memiliki efek sebagai antidiabetes, rerata sebesar 97.49 dan rerata kontrol positif (glibenklamid) sebesar 116.83 pada penurunan kadar glukosa darah pada tikus yang diinduksi streptozotocin. Hasil studi memperlihatkan Berdasarkan analisis One Way ANOVA nilai sig. yakni 0.000 < 0.05, maka simpulannya rata-rata kelima kelompok uji memiliki perbedaan yang bermakna.