Phytochemical Screening and Analysis of Caffeine Content in Arabica Ground Coffee in Takengon City Using Spectrophotometry Ultraviolet / Skrining Fitokimia Dan Analisis Kadar Kafein Pada Kopi Bubuk Jenis Arabika Di Kota Takengon Menggunakan Spektrofotometri Ultraviolet

Background: Coffee is a type of plant that contains caffeine and can be made into a delicious drink. Currently, coffee is the world's most popular drink after water and tea. The stuay aimed was to determine the caffeine content in Arabica ground coffee circulating in the Takengon market according to Indonesian national standards (SNI 01-7152-2006). This research was experimental by taking samples purposively. The research method used was phytochemical screening, qualitative analysis, and ultraviolet spectrophotometry at a wavelength of 273nm. The specified validation parameters were precision, linearity, LOD and LOQ. The results of the phytochemical screening of sample A in the Meyer test treatment obtained negative results but the others were positive. And in sample B, all tests were positive. In the three qualitative analyses tests on samples A and B, the results were positive. The average yield of quantitative analysis in sample A was 11.632 mg once and the daily concentration was 34.897mg. while in sample B the level was 6.663 mg once and drank 19.989mg per day. Validation test results obtained linearity r2 = 0.998, LOD and LOQ namely 0.882µg/ml and 2.94µg/ml, RSD value of 1.11%. Based on the conclusion, sample A and sample B met the requirements according to SNI 2006 (50mg once and 150mg daily) and also met the requirements according to the Pharmacopoeia Edition III, namely a maximum dose of 500 mg once and 1.5g daily.

Latar belakang: Kopi merupakan jenis tumbuhan yang mengandung kafein dan dapat diolah minuman lezat. Saat ini kopi menjadi minuman paling disukai masyarakat dunia setelah air dan teh. Tujuan untuk mengetahui kadar kafein pada kopi bubuk arabika yang beredar di pasaran kota takengon tersebut sesuai standar nasional Indonesia (SNI 01-7152-2006). Jenis penelitian ini eksperimental dengan mengambil sampel secara purposive. Metode penelitian yang dilakukan adalah skrining fitokimia, analisa kualitatif, dan spektrofotometri ultraviolet pada panjang gelombang 273 nm. Parameter validasi yaitu Presisi, lineritas, LOD dan LOQ. Hasil dari skrining fitokimia sampel A pada perlakuan uji meyer mendapatkan hasil negatif tetapi yang lainnya positif. Dan pada sampel B semua pengujian mendapatkan hasil positif. Pada ketiga pengujian analisa kualitatif pada sampel A dan B mendapatkan hasil yang positif. Hasil rata-rata analisa kuantitatif pada sampel A sekali minum 11,632 mg dan kadar sehari 34,897 mg. sedangkan pada sampel B kadar sekali minum 6,663 mg dan sehari minum 19,989 mg. Hasil uji Validasi diperoleh linearitas r2 = 0,998, LOD dan LOQ yaitu 0,882 µg/ml dan 2,94 µg/ml, nilai RSD yaitu 1,11%. Kesimpulan, sampel A dan sampel B memenuhi syarat menurut SNI tahun 2006 (50 mg sekali dan 150 mg sehari) dan juga memenuhi syarat menurut farmakope edisi III yaitu dosis maksimum sekali 500 mg dan sehari 1,5 g