Qualitative Analysis and Determination of Total Flavonoid Content of Ethanolic Extract of Gotu Kola (Centella asiatica) With Variation of Solvent Concentrations / Uji Kualitatif Dan Penetapan Kadar Flavonoid Total Ekstrak Etanol Herba Pegagan (Centella asiatica) Dengan Variasi Konsentrasi Pelarut

Gotu Kola (Centella asiatica) has been known as a medicinal plant that has pharmacological activity in various diseases. One of the many bioactive compounds found is flavonoids. This study aims to determine the effect of concentration on total levels of flavonoids in the ethanol extract of Centella asiatica herb (EEHP). The method of measuring total flavonoid content was UV-Visible spectrophotometry with a maximum lambda of 412 nm and using quercetin as a reference standard. The results of total flavonoid levels in EEHP 50%, EEHP 70%, EEHP 80%, and EEHP 96% were 5.58 mgQE/g; 5.24 mgQE/g; 6.67 mgQE/g; 11.92 mgQE/g. The ANNOVA test showed a significant difference in the results of 96% EEHP flavonoid levels with a sig value of 0.001 (<0.5). Trigger concentration is proven to affect the levels of flavonoids contained in the extract. The highest levels of flavonoids were obtained at 96% EEHP, which is 11.92 mgQE/g.

Pegagan (Centella asiatica) telah dikenal sebagai tanaman obat yang memiliki aktivitas farmakologi dalam berbagai penyakit. Kandungan senyawa bioaktif yang banyak ditemukan salah satunya adalah flavonoid. Penelitian ini bertujuan mengetahui pengaruh konsentrasi pelarut terhadap kadar total flavonoid dalam ekstrak etanol herba pegagan (EEHP). Metode pengukuran kadar total flavonoid adalah spektrofotometri UV-Visibel dengan lambda maksimum 412nm serta menggunakan baku pembanding kuersetin. Hasil kadar flavonoid total pada EEHP 50%, EEHP 70%, EEHP 80%, dan EEHP 96% berturut-turut yaitu 5,58 mgQE/g; 5,24 mgQE/g; 6,67 mgQE/g; 11,92 mgQE/g. Uji ANNOVA menunjukkan perbedaan yang signifikan pada hasil nilai kadar flavonoid EEHP 96% dengan nilai sig 0,001 (<0,5). Konsentrasi pelarut terbukti mempengaruhi kadar flavonoid yang terkadung dalam ekstrak. Kadar flavonoid tertinggi diperoleh pada EEHP 96% yaitu 11,92 mgQE/g.