Efficacy of Nothopanax sculleturium (mangkokan leaf) and Colocasia esculenta L. (taro stalk) ointment in the healing of male rabbit incised wounds. / Uji efektivitas salep kombinasis Nothopnax sculleturium (daun mangkokan) dan Colocasia esculenta L. (daun talas) terhadap penyembuhan luka sayat kelinci jantan

Background: Nothopanax scutellarium (mangkokan leaves) and Colocasia esculenta L. (taro petioles) contain bioactive compounds such as flavonoids, saponins, tannins, terpenoids, and steroids, which play essential roles in wound healing. Objective: This study aimed to evaluate the effectiveness of a topical ointment containing a combination of ethanolic extracts of mangkokan leaves and taro petioles on incised wound healing in male rabbits. Methods: A laboratory experimental study with a post-test-only control group design was conducted using three ointment formulas (10%:5%, 7.5%:7.5%, and 5%:10%), alongside positive (povidone-iodine) and negative controls. Wound length was measured daily for 10 days. Data were analysed using one-way ANOVA followed by Tukey's post-hoc test at a significance level of α = 0.05. Results: All formulations significantly accelerated wound healing compared with the negative control (p < 0.05). The 5%:10% formulation achieved complete wound closure on day 7, showing a statistically significant difference from the positive control (p < 0.05). Conclusion: The combination of mangkokan leaf and taro petiole extracts effectively promotes incised wound healing, with the 5%:10% formula demonstrating the most optimal effect. These findings support the potential of developing this herbal ointment as a safe and efficient antiseptic alternative.

Latar Belakang: Daun mangkokan (Nothopanax scutellarium) dan tangkai daun talas (Colocasia esculenta L.) mengandung flavonoid, saponin, tanin, terpenoid, dan steroid yang berperan dalam proses penyembuhan luka. Tujuan: Mengevaluasi efektivitas kombinasi ekstrak etanol daun mangkokan dan tangkai daun talas dalam sediaan salep terhadap penyembuhan luka sayat pada kelinci jantan. Metode: Penelitian eksperimental post-test only control group design ini menggunakan tiga formula salep (10%:5%, 7,5%:7,5%, dan 5%:10%) dengan kontrol positif (povidone iodine) dan kontrol negatif. Panjang luka diukur setiap hari selama 10 hari. Data dianalisis menggunakan ANOVA satu arah dan uji lanjut Tukey HSD (α = 0,05). Hasil: Semua formula mempercepat penyembuhan luka secara signifikan dibandingkan dengan kontrol negatif (p < 0,05). Formula 5%:10% menunjukkan penyembuhan sempurna pada hari ke-7 dan berbeda secara signifikan dibandingkan dengan kontrol positif (p < 0,05). Kesimpulan: Kombinasi ekstrak daun mangkokan dan tangkai daun talas efektif dalam mempercepat penyembuhan luka sayat, dengan rasio 5%:10% sebagai konsentrasi yang paling optimal. Temuan ini mendukung potensi pengembangan salep herbal sebagai alternatif antiseptik yang aman dan efektif.