Phytochemical Analysis and Potential Test of Ethanol Extract of Matoa (Pometia pinnata L) Stem Bark as an Antibacterial Against the Growth of Escherichia coli / Analisis Fitokimia Dan Uji Potensi Ekstrak Etanol Kulit Batang Matoa (Pometia pinnata L) Sebagai Antibakteri Terhadap Pertumbuhan Escherichia coli

Background: Diarrhea is a health condition, can be caused by Escherichia coli bacteria. Matoa bark has pharmacological activity, can be used as an alternative antibacterial treatment from natural ingredients. Objective: to determine the content of phytochemical compounds and the potential of ethanol extract of matoa bark as an antibacterial against the growth of Escherichia coli. Method: Type of laboratory experiment research, maceration method extraction, 95% ethanol, test solution concentrations of 10%, 20%, 30%. positive control Ciprofloxacin, negative control aquadestilata. Phytochemical analysis based on color reactions and sediment. Antibacterial potential test disc diffusion method, inhibition zone measured using calipers, observation 1x24 hours. Results: Ethanol extract of matoa bark contains phytochemical compounds flavonoids, triterpenoids, saponins, tannins. The extract concentrations of 10%, 20%, and 30% have antibacterial activity with an average inhibition zone diameter of 15.75±0.39 mm, 16.09±0.19 mm, and 18.14±0.73 mm, respectively, which are classified as strong. The diameter of the inhibition zone for Ciprofloxacin is 29.56±0.30 mm. The higher the concentration of the extract, the larger the inhibition zone. Conclusion: The ethanol extract of matoa (Pometia pinnata L) stem bark contains phytochemical compounds flavonoids, triterpenoids, saponins, and tannins. Concentrations of 10%, 20%, and 30% have antibacterial activity against Escherichia coli, which are classified as strong. The best test results were obtained at a concentration of 30%.

Latar belakang: Diare merupakan suatu kondisi kesehatan, dapat disebabkan oleh bakteri Escherichia coli. Kulit batang matoa memiliki aktivitas farmakologis, dapat digunakan sebagai alternatif pengobatan antibakteri dari bahan alam.  Tujuan: mengetahui kandungan senyawa fitokimia dan potensi ekstrak etanol kulit batang matoa sebagai antibakteri terhadap pertumbuhan Escherichia coli. Metode: Jenis penelitian eksperimen laboratorium, ekstraksi metode maserasi, etanol 95%, larutan uji konsentrasi 10%, 20%, 30%. kontrol positif Ciprofloxacin, kontrol negatif aquadestilata. Analisis fitokimia berdasarkan reaksi warna dan endapan. Uji potensi antibakteri metode difusi cakram, zona hambat diukur menggunakan jangka sorong, pengamatan 1x24 jam. Hasil: Ekstrak etanol kulit batang matoa  mengandung senyawa fitokimia flavonoid, triterpenoid, saponin, tanin. Ekstrak konsentrasi 10%, 20%, 30% memiliki aktivitas antibakteri dengan rata-rata diameter zona hambat secara berturut 15,75±0,39 mm, 16,09±0,19 mm dan 18,14±0,73 mm, termasuk kategori kuat. Diamater zona hambat Ciprofloxacin 29,56±0,30 mm. Semakin besar konsentrasi ekstrak, semakin besar zona hambat. Kesimpulan: ekstrak etanol kulit batang matoa (Pometia pinnata L) mengandung senyawa fitokimia flavonoid, triterpenoid, saponin, tanin. Konsentrasi 10%, 20% dan 30% memiliki aktivitas antibakteri terhadap Escherichia coli, kategori kuat. Hasil uji terbaik diperoleh pada konsentrasi 30%.