Effect of Adjunctive Collagen Gel Therapy on Salivary Matrix Metalloproteinase-8 Levels in Gingivitis Following Scaling and Root Planing / Efek Terapi Adjuvan Gel Kolagen terhadap Kadar Matriks Metaloproteinase-8 Saliva pada Gingivitis Pasca Scaling Root Planing

Gingivitis is a reversible inflammatory condition of the gingival tissue without attachment loss, primarily caused by plaque accumulation, which can trigger an immune response. Matrix metalloproteinase-8 (MMP-8) is an enzyme that contributes to collagen degradation. Scaling and root planing (SRP) is the standard therapy; however, it may induce a transient inflammatory response and result in suboptimal tissue regeneration, thereby necessitating adjunctive therapy. This study aimed to evaluate the effect of adjunctive collagen gel therapy on salivary matrix metalloproteinase-8 levels in gingivitis. This experimental study employed a pre- and post-test control group design involving 50 subjects divided into five groups: healthy control, gingivitis treated with SRP, and gingivitis treated with SRP combined with 1%, 2%, and 4% collagen gel. Salivary MMP-8 levels were measured to assess the inflammatory response. Statistical analysis revealed that collagen gel at concentrations of 1% and 2% had a significant effect on MMP-8 levels. In conclusion, Collagen gel at 1% and 2% concentrations significantly modulated salivary MMP-8 levels, suggesting a potential role in the tissue remodeling phase following SRP.

Gingivitis merupakan kondisi inflamasi reversibel pada jaringan gingiva tanpa kehilangan perlekatan yang terutama disebabkan oleh akumulasi plak sehingga dapat memicu respon imun. Matriks metaloproteinase-8 merupakan salah satu enzim yang berkontribusi dalam memecah kolagen. Scaling and root planing merupakan terapi standar, namun dapat menimbulkan respons inflamasi sementara dan regenerasi jaringan yang kurang optimal, sehingga diperlukan terapi adjuvan. Penelitian ini bertujuan untuk mengevaluasi efek terapi adjuvan gel kolagen terhadap kadar matriks metaloproteinase-8 saliva pada gingivitis. Penelitian ini merupakan studi eksperimental dengan rancangan pre- dan post-test control group desain yang melibatkan 50 subjek yang dibagi menjadi lima kelompok, yaitu kelompok sehat, gingivitis dengan SRP, serta gingivitis dengan SRP yang dikombinasikan dengan gel kolagen konsentrasi 1%, 2%, dan 4%. Kadar MMP-8 saliva diukur untuk menilai respons inflamasi. Hasil uji statistic terdapat efek yang signifikan kolagen gel 1% dan 2% terhadap MMP-8. Gel kolagen 1% dan 2% memodulasi kadar MMP-8 saliva, yang mengindikasikan potensi peran dalam fase remodeling pasca SRP.