Nephroprotective Effect of Butterfly Pea Flower (Clitoria ternatea L.) Extract in a Rat Model of Nephrotoxicity: Biochemical, Histopathological, Antioxidant, and Cytokine, Analyses / Efek Nefroprotektif Ekstrak Bunga Telang (Clitoria ternatea L.) Pada Tikus Model Nefrotoksisitas: Analisis Biokimia, Histopatologi, Antioksidan, dan Sitokin

Background: Butterfly pea flower (Clitoria ternatea L.) is rich in antioxidants and bioactive compounds that may protect the kidney from nephrotoxic injury. Objective: This study aimed to evaluate the nephroprotective effect of ethanolic extract of butterfly pea flower in gentamicin-induced rats. Methods: This in vivo experimental study used 30 male Wistar rats divided into five groups: normal control, negative control, and three treatment groups receiving butterfly pea flower extract at doses of 100, 200, and 400 mg/kgBW. The extract was administered orally, while gentamicin 100 mg/kgBW was given intraperitoneally. Parameters observed included BUN, urea, IL-6, TNF-α, renal morphology, kidney weight, and histopathology. Results: Gentamicin increased BUN, urea, IL-6, and TNF-α levels and caused histopathological damage and morphological changes in the kidney. Administration of butterfly pea flower extract reduced inflammatory response, improved histopathology, and restored renal morphology and kidney weight toward normal. The best histopathological improvement was observed at 200 mg/kgBW, whereas the strongest anti-inflammatory effect was found at 400 mg/kgBW. Conclusion: Ethanolic extract of butterfly pea flower has potential as a nephroprotective agent against gentamicin-induced nephrotoxicity.

Latar belakang: Bunga telang (Clitoria ternatea L.) kaya akan antioksidan dan senyawa bioaktif yang berpotensi melindungi ginjal dari efek nefrotoksik. Tujuan: Mengetahui efek nefroprotektif ekstrak etanol bunga telang pada tikus yang diinduksi gentamisin. Metode: Penelitian eksperimental in vivo ini menggunakan 30 tikus jantan Wistar yang dibagi menjadi lima kelompok, yaitu kontrol normal, kontrol negatif, dan tiga kelompok perlakuan ekstrak bunga telang dosis 100, 200, dan 400 mg/kgBB. Ekstrak diberikan secara oral, sedangkan gentamisin 100 mg/kgBB diberikan secara intraperitoneal. Parameter yang diamati meliputi BUN, ureum, IL-6, TNF-α, morfologi, berat, dan histopatologi ginjal. Hasil: Gentamisin meningkatkan BUN, ureum, IL-6, dan TNF-α serta menyebabkan kerusakan histopatologi dan perubahan morfologi ginjal. Pemberian ekstrak bunga telang menurunkan respons inflamasi, memperbaiki histopatologi, dan mendekatkan morfologi serta berat ginjal ke kondisi normal. Perbaikan jaringan paling baik terlihat pada dosis 200 mg/kgBB, sedangkan efek antiinflamasi paling kuat tampak pada dosis 400 mg/kgBB. Kesimpulan: Ekstrak etanol bunga telang berpotensi sebagai agen nefroprotektif terhadap nefrotoksisitas akibat gentamisin.