Profile of secondary metabolite compounds of n-hexane and ethyl acetate fractions of clove leaves (Syzygium aromaticum L.) using LC-HRMS / Profil senyawa metabolit sekunder dari fraksi n-heksana dan etil asetat daun cengkeh (Syzygium aromaticum L.) menggunakan LC-HRMS

The diversity of plants available in nature, the majority have the potential as a source of traditional medicine due to the presence of secondary metabolites in various parts of the plant, especially the leaves. The object of the study was clove leaves (Syzygium aromaticum L.), a plant species abundant in secondary metabolites including alkaloids, flavonoids, tannins, saponins, and steroids/triterpenoids. This research aims to compare the profile of secondary metabolites in clove leaves (Syzygium aromaticum L.) in the n-hexane and ethyl acetate fractions. The research methodology includes phytochemical screening of secondary metabolites of the n-hexane and ethyl acetate fractions of clove leaves (Syzygium aromaticum L.) with dye reagents and LC-HRMS analysis. Phytochemical screening of the n-hexane fraction confirmed the presence of steroids, while the ethyl acetate fraction confirmed flavonoids, tannins, and steroids. The results of identification using the LC-HRMS instrument on the N-Hexane and Ethyl Acetate fractions of clove leaves (Syzygium aromaticum L) using LC-HRMS contained phenolic, flavonoid and phenylpropanoid compounds. In the results of the chromatogram in the N-Hexane fraction, Rhamnetin compound was obtained which had an area percent of 0.362% with the highest area of 10954249. Meanwhile, in the results of the ethyl acetate fraction chromatogram, eugenol compounds were obtained with an area percent of 0.37% with the highest area of 33151358.

Keberagaman tanaman yang tersedia di alam, mayoritas memiliki potensi sebagai sumber obat tradisional karena kehadiran metabolit sekunder dalam berbagai bagian tanaman, khususnya daun. Objek penelitian adalah daun cengkeh (Syzygium aromaticum L.), spesies tumbuhan yang berlimpah metabolit sekunder mencakup alkaloid, flavonoid, tanin, saponin, dan steroid/triterpenoid. Riset ini bertujuan membandingkan profil metabolit sekunder dalam daun cengkeh (Syzygium aromaticum L.) pada fraksi n-heksana dan etil asetat. Metodologi penelitian meliputi skrining fitokimia metabolit sekunder fraksi n-heksana dan etil asetat daun cengkeh (Syzygium aromaticum L.) dengan reagen pewarna dan analisis LC-HRMS. Skrining fitokimia fraksi n-heksana mengkonfirmasi keberadaan steroid, sedangkan fraksi etil asetat mengkonfirmasi flavonoid, tanin, dan steroid. Hasil identifikasi menggunakan instrumen LC-HRMS pada fraksi N-Heksan dan Etil Asetat daun cengkeh (Syzygium aromaticum L) menggunakan LC-HRMS terdapat senyawa fenolik, flavonoid dan phenilpropanoid. Pada hasil kromatogram pada fraksi N-Heksan diperoleh senyawa Rhamnetin yang memiliki persentase are sebesar 0,362% dengan area tertinggi sebesar 10954249. Sedangkan pada hasil kromatogram fraksi etil asetat diperoleh senyawa eugenol memiliki persentase are sebesar 0,37% dengan area tertinggi sebesar 33151358