Formulation and Testing of Antibacterial Activity of Liquid Soap Preparations Ethanol Extract of Arabika Coffee Leaves (Coffea arabica L.) Against the Bacteria Staphylococcus aureus / Formulasi dan Uji Aktivitas Antibakteri Sediaan Sabun Cair Ekstrak Etanol Daun Kopi Arabika (Coffea arabica L.) Terhadap Bakteri Staphylococcus aureus

Coffee leaves (Coffea arabica L.) are a part of the coffee plant that is not widely used. Coffee leaves contain flavonoids, alkaloids, saponins, caffeine and polyphenols. Coffee leaves are a natural ingredient that has antibacterial and antioxidant activity, so they need to be used. develop it into pharmaceutical preparations to increase ease of use, one of which is liquid soap preparations The aim of this research was to determine the secondary metabolite content of simplicia powder and ethanol extract of coffee leaves, formulate ethanol extract of coffee leaves into liquid soap, determine the physical quality of the preparation and test the activity against Staphylococcus aureus bacteria. The method used in this research is to make a liquid soap formulation from Arabica coffee leaves and test its effectiveness as an antibacterial against Staphylococcus aureus bacteria. This research was carried out experimentally with the independent variable being the concentration of Arabica coffee leaf ethanol extract: F1 contains 2.5 grams of active substance, F2 contains 5.0 grams of active substance, F3 contains 7.5 grams of active substance, dependent variable: leaf simplicia phytochemical screening test Arabica coffee, antibacterial activity test against Staphylococcus aureus bacteria. Quality of liquid soap preparations from ethanol extract of Arabica coffee leaves using stability tests (pH of the preparation, homogeneity, viscosity and height of foam) and antibacterial tests using the agar diffusion method (well method). The research results show that simplicia powder and ethanol extract of coffee leaves contain alkaloid, flavonoid, tannin and saponin chemical compounds. The organoleptic test results of liquid soap have a distinctive smell, a combination of coffee and rose leaves, are blackish brown in color, and are in the form of a thick liquid. The stability test results of all formulas are stable. The pH test ranges from 9.05 to 9.43. Test foam height 35- 40 mm. The viscosity test results ranged from 735.0-894.8 cpoise, antibacterial activity had an k- (Blanko) concentration inhibition zone of  13,6 mm FI concentration liquid soap obtained a result of 15.7 mm, FII concentration liquid soap obtained a result of 18.4 mm, FIII concentration liquid soap obtained a result of 18.75 mm compared to the control positive obtained a result of 18.8 mm.

Daun Kopi (Coffea arabica L.) merupakan salah satu bagian dari tanaman kopi yang belum banyak dimanfaatkan, Daun kopi mengandung flavonoid, alkaloid, saponin, kafein, dan polifenol, Daun kopi merupakan bahan alam yang memiliki aktivitas antibakteri dan antioksidan, sehingga perlu di kembangkan menjadi sediaan farmasi untuk meningkatkan kemudahan dalam penggunaan nya, salah satu nya adalah sedian sabun cair. Tujuan dari penelitian ini adalah untuk mengetahui kandungan metabolit sekunder simplisiadaun kopi, memformulasikan ekstrak etanol daun kopi menjadi sabun cair, mengetahui mutu fisik sediaan dan menguji aktivitas terhadap bakteri Staphylococcus aureus. Metode yang digunakan pada penelitian ini membuat formulasi sabun cair dari daun kopi arabika dan menguji efektifitasnya sebagai antibakteri terhadap bakeri Staphylococcus aureus. Penelitian ini dilakukan secara eksperimental dengan variabel bebas konsentrasi ekstrak etanol daun kopi arabika : F1 mengandung zat aktif 2,5 gr,  F2 mengandung zat aktif 5,0 gr, F3 mengandung zat aktif 7,5 gr, variabel terikat:  uji skrining fitokimia simplisia daun kopi arabika, uji aktivitas antibakteri terhadap bakteri Staphylococcus aureus. Mutu sediaan sabun cair ekstrak etanol daun kopi arabika  dengan uji stabilitas (pH sediaan , homogenitas ,viskositas dan ketinggian busa ) dan uji antibakteri dengan metode difusi agar (cara sumuran). Dari hasil penelitian menunjukkan bahwa simplisia daun kopi mengandung senyawa kimia alkaloid, flavonoid, tanin, dan saponin. Hasil uji Hasil uji stabilitas dari semua formula stabil. Uji pH berkisar 9,05-9,43. Uji tinggi busa 70-80 mm. Uji viskositas hasilnya berkisar 735,0-894,8 cpoise, aktivitas antibakteri mempunyai zona hambat konsentrasi k- (Blanko) di peroleh 13,6 mm, FI diperoleh yaitu 15,7  mm, sabun cair konsentrasi FII diperoleh hasil 18,4 mm, sabun cair konsentrasi FIII diperoleh hasil 18,75 mm pembanding kontrol positif diperoleh hasil 18,8 mm.