Kelubi fruit (Eleiodoxa conferta (Griff.) Burret) is a fruit plant that contains flavonoid compounds that are very beneficial for the skin. The novelty of this study is that researchers tested the effectiveness of antioxidants using UV-visible spectrophotometry on the formulation of ethanol extract of kelubi fruit flesh in skin moisturizing cream preparations. The purpose of the study was to analyze ethanol extract of kelubi fruit flesh formulated into a moisturizing cream preparation at several concentration variations that have activity as antioxidants and do not cause irritation to the skin. The study used descriptive and experimental methods with test materials for kelubi fruit flesh which was macerated using 96% ethanol filtering formulated into a moisturizing cream utilizing a concentration of 1.5%, 2.0%, 2.5%, and blanks. The test results on moisturizing cream preparations showed that ethanol extract of kelubi fruit flesh (Eleiodoxaconferta (Griff.) Burret) can be formulated in the dosage form of a homogeneous moisturizing cream with an M / A emulsion type and has a pH value that is under the pH of human physiological skin, namely the pH value of the moisturizing cream preparation shortly after it is made is 6.1-6.5, and the pH of the moisturizing cream preparation after the cycling test is 6.0-6.3. A moisturizing cream preparation with a concentration of ethanol extract of kelubi fruit flesh 2.5% is the best preparation that can provide moisture to the skin of volunteers with a value of 56 where the value is categorized as "Hydration," with a moisture recovery percent on the skin of 28.04% for 4 weeks of use with a moderate antioxidant category (IC50 = 118.4903 ppm) and blanks classified as very weak antioxidants (IC50 = 243.0549 ppm). In conclusion, preparing the moisturizing cream of ethanol extract from kelubi fruit flesh does not cause an irritating effect on the skin.